Melissa Mattocks, B.A., Lab Manager
Melissa Mattocks joined the GVI team in May 2011 along with Dr. Laura White. She worked with Dr. White at the Carolina Vaccine Institute at the University of North Carolina at Chapel Hill since the conception of the dengue vaccine project, moving with Dr. White to GVI in 2011. Ms. Mattocks has played a central role setting up the virological and immunological assays, and the animal models required for the construction and testing of a dengue vaccine. Her wide range of technical expertise includes molecular biology, cloning dengue genes into the alphavirus vectors and production of transcripts for RNA electroporation; alphavirus replicon particles production and purification under BSL-3 and BSL-2 containment laboratory conditions; dengue virus growth, purification and characterization; optimization and performance of immunological assays including a flowcytometry-based virus neutralization assay; and mouse colony management and immunizations, bleedings and virus challenges.
A.A., Lenoir Community College, Liberal Arts, 1991
B.A., Biology, University of North Carolina at Chapel Hill, 2010
Lab Manager, Global Vaccines, Inc., May 2013-present
Research Assistant III, Global Vaccines, Inc., 2011-2013
Research Specialist III, Carolina Vaccine Institute, 2009-2011
Research Specialist II, Carolina Vaccine Institute, 2008-2009
Research Technician III, Carolina Vaccine Institute, 2007-2008
Research Technician II, Carolina Vaccine Institute, 2005-2007
Lab Assistant, University of North Carolina at Chapel Hill, Dept. of Microbiology, 2003-2005
- White Laura J, Parsons Melissa Mattocks, Whitmore Alan C, Williams Brandon M, de Silva Aravinda, Johnston Robert E, An immunogenic and protective alphavirus replicon particle-based dengue vaccine overcomes maternal antibody interference in weanling mice. J Virol. 2007 Oct;81(19):10329-39.
- Heise Mark T, Whitmore Alan, Thompson Joseph, Parsons Melissa Mattocks, et.al. An alphavirus replicon-derived candidate vaccine against Rift Valley fever virus. Epidemiol Infect. 2009 Sep;137(9):1309-18.
Molecular cloning; PCR; DNA isolation, purification, and production; facs-based virus neutralization assays; SDS-Page; Western blot; in vitro transcription of RNA; mammalian and insect tissue culture; bacterial culture; virus production; plaque assay; VEE replicon particle production, purification, and infection; VRP titration; electroporation; BSL-2 and BSL-3 research; IFA; flow cytometry (Accuri C6, FacScan, and Cyan); mouse husbandry, serum collection, and immunization; ELISA